The object is to deliver the CO2 into the container in a very slow & steady stream that will cause the rodents to go to sleep and eventually suffocate in their sleep, not knowing or feeling a thing.
With the rodents in the container and lid in place, slowly turn CO2 valve on a little at a time with micro-adjustments each time. If the CO2 goes into the container too fast it will cause the rodents to choke from lack of oxygen and cause them to suffer. It's not a pretty sight.
If done properly the rodents will gently lay their heads down and go to sleep. If they start gasping, as if choking, or anything else that looks like they could be suffering instead of dying a peaceful death, reduce the flow of CO2 into the container until they settle down peacefully.
When all of the rodents appear to be dead increase the flow of CO2 for a few seconds in order to flood the chamber. Wait 5 - 10 minutes to make sure they're all dead before removing the lid.
If you will be freezing any of the rodents I suggest letting them cool to room temperature before bagging them and putting them in the freezer. Remove all air that you can from the bag before sealing it & placing it in the freezer. These steps will reduce the ice buildup on their bodies and will increase their freezer storage time.
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Last updated: 11/27/07